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KMID : 0900920010250030287
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Korean journal of Animal Reproduction
2001 Volume.25 No. 3 p.287 ~ p.292
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Chromosome and Spindle Configuration of Mouse Oocytes after Vitrification at the Mature Stage
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Ko Jeong-Jae
Chung Hyung-Min
Gary B. Anderson
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Abstract
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Selection of oocyte cryopreseivation method is a prerequisite factor for developing an effective bank system. To develop an effective vitrification method, we examined whether damages in spindle and chromosome morphology induced by vitrification. Intact cumulus-enclosed oocytes were vitrified with DPBS with 5.5 M ethylene glycol and 1.0 M sucrose, and loaded onto eletron microscopic copper grid for storing in liquid nitrogen. Intact vitrified and thawed oocytes were immunostaining for tubulin and karyotying for chromosome. Vitrfied and thawed oocytes had a higher rate of chromosome (32.8% vs. 19.6%) and spindle (32.3% vs. 20.2%) abnormalities compared with fresh oocytes. Mouse oocytes after vitrification at the mature stage showed increased incidence of chromosomal and spindle abnormalities.
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KEYWORD
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Vitrification, Chromosome, Spindle, Immunostaining
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